Product Description

Histone H2B (Acetyl Lys24/25) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES8626-50, ES8626-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: H2BFS, Histone H2B type F-S, Histone H2B.s, H2B/s, H2BK2425AC

Clonality: Polyclonal

Immunogen: Synthetic Acetyl peptide from human protein at AA range: 24/25

Isotype: IgG

Target: Histone H2B

Specificity: The antibody detects endogenous Histone H2B when Acetyl occurs at Lys24 or 25)

Dilution: WB 1:500-2000, ELISA 1:10000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level), Also found in some fat cells,

Observed Band (KD): 15kD

Fields: Neutrophil extracellular trap formation, Alcoholism, Viral carcinogenesis, Systemic lupus erythematosus

Function: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
PTM: Monoubiquitination of Lys-122 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II
Similarity: Belongs to the histone H2B family
Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: Histone H2B

Protein Name: Histone H2B

Research Use Only