Product Description

Histone H2B (Di Methyl Lys43) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20560-50, ES20560-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: H2BK43ME2, HIST1H2BA, TSH2B, Histone H2B type 1-A, Histone H2B, testis, Testis-specific histone H2B, HIST1H2BB, H2BFF, Histone H2B type 1-B, Histone H2B.1, Histone H2B.f, H2B/f, HIST1H2BC, H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK, Histone H2B type 1-C/E/F/G/I, Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l

Clonality: Polyclonal

Immunogen: Synthetic Peptide of Histone H2B (Di Methyl Lys43)

Isotype: IgG

Target: Histone H2B

Specificity: The antibody detects endogenous Histone H2B (Di Methyl Lys43) protein.

Dilution: WB: 1:500-1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Expression: Mainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level), Also found in some fat cells,

Observed Band (KD): 14kD

Fields: Neutrophil extracellular trap formation, Alcoholism, Viral carcinogenesis, Systemic lupus erythematosus

Function: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
PTM: Monoubiquitination of Lys-122 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II
Similarity: Belongs to the histone H2B family
Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: HIST1H2BC

Protein Name: Histone H2B type 1-A/Histone H2B type 1-B/Histone H2B type 1-C/E/F/G/I

Mouse Gene ID: 319177/319178/319179

Rat Gene ID: 24829

Rat SwissProt Number: Q00729

Research Use Only