Product Description

Histone H3 (Acetyl Lys123) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1134-50, ES1134-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST2H3A, HIST2H3C, H3F2, H3FM, HIST2H3D, Histone H3.2, Histone H3/m, Histone H3/o, H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B, Histone H3.3, H3F3C, Histone H3.3C, Histone H3.5, H3k123AC

Clonality: Polyclonal

Immunogen: Synthesized acetyl-peptide derived from the C-terminal region of human Histone H3 around the acetylation site of K123.

Isotype: IgG

Target: Histone H3

Specificity: Acetyl-Histone H3 (K123) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylation at K123.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Blood, Epithelium, Kidney, Lung, Ovary, Spleen, Uterus,

Observed Band (KD): 17kD

Fields: Neutrophil extracellular trap formation, Alcoholism, Shigellosis, Transcriptional misregulation in cancer, Systemic lupus erythematosus

Function: Caution: Was originally (PubMed: 2587222) thought to originate from mouse
Developmental Stage: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation
Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
mass spectrometry: Monoisotopic with N-acetylserine PubMed: 16457589,Miscellaneous: This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2)
PTM: Acetylation is generally l

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C

Protein Name: Histone H3.1/Histone H3.2/Histone H3.3/Histone H3.3C

Mouse Gene ID: 319152/15077/15078/625328

Rat Gene ID: 291159/100361558

Rat SwissProt Number: Q6LED0/P84245

Research Use Only