Histone H3 Rabbit mAb
Size: 100μg
Catalogue Number: AC070-100
Citations, Manuals and MSDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Category: Loading Control Antibodies
Applications: WB, IP, IHC-P, ChIP, ELISA
Cross-reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Protein Weight: 15 kDa
Observed Molecular Weight: 17 kDa
Immunogen: Synthetic peptide
Species: Human
GeneID: 8290/8350
SWISS: Q16695/P68431
Alternate Names: H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Histone H3; H3-4; H3/t; H3/g; H3FT; HIST3H3
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:80000 - 1:320000 IP, 0.5μg-4μg antibody for 100μg-300μg extracts of whole cells IHC-P, 1:5000 - 1:20000 ChIP, 2μg antibody for 10μg-15μg of Chromatin ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (≥1:10000) ，a sequential dilution method is strongly recommended to ensure measurement accuracy.
Research Areas: Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Cell Cycle, Immunology Inflammation, NF-kB Signaling Pathway.
NCBI Alias: H3C1/H3-4
Clone Number Info: ARC75100
Research Use Only