Histone H3 Rabbit pAb
Sizes: 20µl, 100µl
Catalogue Numbers: A2348-20, A2348-100
Citations, Manuals and SDS Available upon request.
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Synonyms: H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
GeneID: 8290/8350
Swiss-Prot: Q16695/P68431
Reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
Tested Applications: ELISA, WB, IHC-P, IF/ICC, IP, ChIP
Calculated Molecular Weight: 16kDa
Observed Molecular Weight: 17kDa
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
Immunogen Species: Human
Host: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage Buffer: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Signal Transduction, MAPK-Erk Signaling Pathway
Recommended Dilutions: WB: 1:2000 - 1:10000 | IHC-P: 1:50 - 1:200 | IF/ICC: 1:50 - 1:200 | IP: 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells | ChIP: 3μg antibody for 5μg-10μg of Chromatin
Modifications: Unmodified
Conjugate: Unconjugated
Cellular Location: Chromosome, Nucleus
Positive Samples: 293F, NIH/3T3, PC-12
Keywords: 8290, HIST3H3, H3.4, H3/g, H3FT, H3t, histone cluster 3 H3, histone H3.1t, H3 histone family, member T, H3/t, histone 3, H3, Q16695, Histone Cluster 3 H3, H3 Histone Family, Member T, Histone 3, H3/G, H3/T, Histone Cluster 3, Histone H3.1t, Histone H3, HIST1H3A, HIST1H3J, H3/j, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l, marker, Chromatin, Chromatin marker
Symbol: H3-4
Category: Polyclonal Antibodies
Research Use Only