HLA-A+HLA-B+HLA-C Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A27768-20, A27768-100
Citations, Manuals and MSDS Available upon request.
Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, ELISA
Cross-reactivity: Human
Protein Weight: 40kDa
Observed Molecular Weight: 40-50kDa
Immunogen: Synthetic peptide .
Species: Human
GeneID: 3105/3106/3107
SWISS: P04439/P01889/P10321
Alternate Names: AS; HLAB; B-4901
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:3000 - 1:12000 IHC-P, 1:500 - 1:1000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Immunology Inflammation.
NCBI Alias: HLA-B
Research Use Only