HLA-DQB1 Polyclonal Antibody - E-AB-92229

HLA-DQB1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-92229-60, E-AB-92229-120, E-AB-92229-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: HLA-DQB1

Target Synonym: IDDM1; CELIAC1; HLA-DQB

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P01920

Background: HLA-DQB1 belongs to the HLA class II beta chain paralogs. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains six exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. Alternative splicing results in multiple transcript variants.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human HLA-DQB1

Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW: 29kDa

Observed MW: 30kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only