Product Description

HLAE Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11907-50, ES11907-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008],

Reactivity: Human

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: HLAE

Specificity: HLAE Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Expressed in secretory endometrial cells during pregnancy (at protein level), The expression in nonlymphoid tissues is restricted to endothelial cells from all types of vessels, including arteries, veins, capillaries, and lymphatics (at protein level), In lymphoid organs, it is mainly expressed in endothelial venules, B and T cells, monocytes, macrophages, NK cells and megakaryocytes (at protein level),

Observed Band (KD): 39kD

Fields: Endocytosis, Phagosome, Cellular senescence, Cell adhesion molecules, Antigen processing and presentation, Type I diabetes mellitus, Human cytomegalovirus infection, Human papillomavirus infection, Human T-cell leukemia virus 1 infection, Kaposi sarcoma-associated herpesvirus infection, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Human immunodeficiency virus 1 infection, Viral carcinogenesis, Autoimmune thyroid disease, Allograft rejection, Graft-versus-host disease, Viral myocarditis

Function: Caution: The existence of allele E*0102 (PubMed: 3260916) and allele E*0104 (PubMed: 1977695) is uncertain. The alleles could not be confirmed in further studies (PubMed: 12445303)
Function: Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules
polymorphism: The following alleles of E-1 are known: E*0101, E*0102, E*0103 and E*0104. The sequence shown is that of E*0101
Similarity: Belongs to the MHC class I family
Similarity: Contains 1 Ig-like C1-type (immunoglobulin-like) domain
Subunit: Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin)

Subcellular Location: Cell membrane, Single-pass type I membrane protein Golgi apparatus membrane, [Soluble HLA class I histocompatibility antigen, alpha chain E]: Secreted

Storage and Stability: -20°C/1 year

Gene Name: HLA-E HLA-6.2 HLAE

Protein Name: HLA class I histocompatibility antigen, alpha chain E (MHC class I antigen E)

Research Use Only