Product Description

Horse ADP (Adiponectin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0636-48, ELK0636-96, ELK0636-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Horse

Alternate Names: GBP28, ApM1, AdipoQ, Acrp30, ACDC, APM1, C1Q And Collagen Domain Containing, Adipocyte Complement-Related Protein Of 30 KDa, Adipose Most Abundant Gene Transcript 1

Sensitivity: 0.053 µg/mL

Assay Type: Sandwich

Standard: 10 µg/mL

Detection Range: 0.16-10 µg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolic Pathway, Infection Immunity, Cardiovascular Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse ADP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only