Horse COL1 (Collagen Type I) ELISA Kit - ELK8222

Horse COL1 (Collagen Type I) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8222-48, ELK8222-96, ELK8222-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: COL1; COLI

Assay Type: Competitive Inhibition

Sensitivity: 7.29 ng/mL

Standard: 1500 ng/mL

Detection Range: 23.44-1500 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Developmental science; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only