Horse COL3 (Collagen Type III) ELISA Kit - ELK8221

Horse COL3 (Collagen Type III) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8221-48, ELK8221-96, ELK8221-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Collagen Type 3; Col 3; Collagen Type III; Col III

Assay Type: Competitive Inhibition

Sensitivity: 4.87 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Cardiovascular biology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only