Product Description

Horse GIP (Gastric Inhibitory Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11862-48, ELK11862-96, ELK11862-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Horse

Alternate Names: Incretin hormone, Glucose Dependent Insulinotropic Peptide

Sensitivity: 23.1 pg/mL

Assay Type: Competitive Inhibition

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 2h

Research Area: Endocrinology, Gastroenterology, Hormone Metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse GIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse GIP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only