Human 2,3-DPG (2,3-Diphosphoglycerate) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0778-48, ELK0778-96, ELK0778-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 2,3DPG
Assay Type: Sandwich
Sensitivity: 0.14 µmol/mL
Standard: 20 µmol/mL
Detection Range: 0.32-20 µmol/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Areas: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only