Product Description

Human 25-OH-D (25 Hydroxy Vitamin D) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0694-48, ELK0694-96, ELK0694-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 25 Hydroxy Vitamin D,

Reactivity: Human

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Reproductive Science, Genetic Science, Nutrition Metabolism, Bone Metabolism

Assay Length: 2.5h

Sensitivity: 1.45 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Research Use Only