Human 5-MTHF (5-Methyltetrahydrofolate) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0833-48, ELK0833-96, ELK0833-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: L-5-MTHF; 5-MTHF;
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction; Reproductive science; Genetic science; Nutrition metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 5-MTHF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-MTHF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 5-MTHF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-MTHF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P49914
Research Use Only