Human ACV-A (Activin A) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0174-48, ELK0174-96, ELK0174-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ACVA; Activin Beta A Beta A Homodimer
Assay Type: Sandwich
Sensitivity: 4.71 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only