Product Description

Human aEP (Alpha-Endorphin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2087-48, ELK2087-96, ELK2087-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aEP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: AEP

Reactivity: Human

Sample Type: Serum, Plasma, Cell Culture Supernatant and other biological fluid

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction, Endocrinology, Neuro Science, Hormone Metabolism

Assay Length: 2h

Sensitivity: 4.51 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Research Use Only