Human AFF1 (AF4/FMR2 Family, Member 1) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3545MS-48, ELK3545MS-96, ELK3545MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: PLA2G2D; sPLA2S; s-PLA2; sPLA2; SPLASH; Secreted Phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Areas: Enzyme and Kinase; Metabolic pathway; Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLA2G2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLA2G2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLA2G2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLA2G2D in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9WVF6
Research Use Only