Human anti-21-OH Ab (anti 21-hydroxylase antibody) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10071-48, ELK10071-96, ELK10071-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 21-OH Ab
Assay Type: Sandwich
Sensitivity: 0.189 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma,Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 21-OH Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 21-OH Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 21-OH Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 21-OH Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only