Human anti-21-OH Ab (anti 21-hydroxylase antibody) ELISA Kit - ELK10071

Human anti-21-OH Ab (anti 21-hydroxylase antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10071-48, ELK10071-96, ELK10071-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 21-OH Ab

Assay Type: Sandwich

Sensitivity: 0.189 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma,Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 21-OH Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 21-OH Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 21-OH Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 21-OH Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only