Human Anti-FOLR (folate receptor autoantibodies IgG) ELISA Kit - ELK0865

Human Anti-FOLR (folate receptor autoantibodies IgG) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0865-48, ELK0865-96, ELK0865-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ANTI-FOLR1

Assay Type: Sandwich

Sensitivity: 3.3 ng/mL

Standard: 500 ng/mL

Detection Range: 7.82-500 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-FOLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-FOLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-FOLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-FOLR in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only