Human Anti-GAD65 (Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0506-48, ELK0506-96, ELK0506-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: anti-GAD65; anti-Pancreatic Islets And Brain,65kDa; anti-65 kDa glutamic acid decarboxylase; anti-Glutamate decarboxylase 65 kDa isoform
Assay Type: Sandwich
Sensitivity: 1.26 IU/mL
Standard: 250 IU/mL
Detection Range: 3.91-250 IU/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Enzyme and Kinase; Endocrinology; Neuroscience; Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GAD65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GAD65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD65 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only