Product Description

Human Anti-GAD65 (Anti-Glutamate Decarboxylase 65 Autoantibodies) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0506-48, ELK0506-96, ELK0506-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GAD65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GAD65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GAD65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GAD65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: anti-GAD65, anti-Pancreatic Islets And Brain, 65kDa, anti-65 kDa glutamic acid decarboxylase, anti-Glutamate decarboxylase 65 kDa isoform

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Endocrinology, Neuro Science, Autoimmunity

Assay Length: 3.5h

Sensitivity: 1.26 IU/mL

Standard: 250 IU/mL

Detection Range: 3.91-250 IU/mL

Research Use Only