Human Anti-GM1 (Anti-Ganglioside Antibody) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0676-48, ELK0676-96, ELK0676-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: anti-ganglioside (GM1) antibody (IgG)
Assay Type: Sandwich
Sensitivity: 0.56 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Areas: Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only