Human Anti-HPF4 (Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit - ELK8037

Human Anti-HPF4 (Anti-Heparin/Platelet Factor 4 Antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8037-48, ELK8037-96, ELK8037-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HPF4 Ab

Assay Type: Competitive Inhibition

Sensitivity: 0.58 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2.5h

Research Areas: Hematology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only