Human Anti-MBL (Anti-Mannose Binding Lectin Antibody) ELISA Kit - ELK8057

Human Anti-MBL (Anti-Mannose Binding Lectin Antibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8057-48, ELK8057-96, ELK8057-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin (Protein C)2,Soluble (Opsonic Defect)

Assay Type: Sandwich

Sensitivity: 2.54 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction; Infection immunity; Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBL in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only