Product Description

Human Anti-OV Ab (Anti-Ovarian Antibody) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0729-48, ELK0729-96, ELK0729-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-OV Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-OV Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-OV Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-OV Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Ovarian antibody

Reactivity: Human

Sample Type: Serum, Plasma and biological fluids

Assay Type: Sandwich

Research Areas: Infection Immunity

Assay Length: 3.5h

Sensitivity: 0.49 U/mL

Standard: 100 U/mL

Detection Range: 1.57-100 U/mL

Research Use Only