Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0752-48, ELK0752-96, ELK0752-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Anti-Tissue Transglutaminase IgA
Assay Type: Sandwich
Sensitivity: 1.45 IU/mL
Standard: 300 IU/mL
Detection Range: 4.69-300 IU/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Enzyme and Kinase; Endocrinology; Neuroscience; Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only