Product Description

Human AP12 (Apelin 12) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1635-48, ELK1635-96, ELK1635-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: APLN12

Reactivity: Human

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Endocrinology, Cardiovascular Biology, Gastroenterology, Hormone Metabolism

Assay Length: 2h

Sensitivity: 25.28 pg/mL

Standard: 6000 pg/mL

Detection Range: 93.75-6000 pg/mL

Research Use Only