Human AP36 (Apelin 36) ELISA Kit - ELK10383

Human AP36 (Apelin 36) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10383-48, ELK10383-96, ELK10383-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: APLN36

Assay Type: Competitive Inhibition

Sensitivity: 0.071 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Endocrinology; Gastroenterology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP36. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP36. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only