Product Description

Human BAFF/CD257 (B-Cell Activating Factor) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2145-48, ELK2145-96, ELK2145-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CD257, TNFSF13B, BLYS, TALL1, THANK, TNFSF20, ZTNF4, B lymphocyte stimulator, Tumor Necrosis Factor Ligand Superfamily Member 13B, Dendritic cell-derived TNF-like, BAFF

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cd and Adhesion Molecule, Tumor Immunity, Infection Immunity, Immune Molecule

Assay Length: 3.5h

Sensitivity: 28 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

UniProt: Q9Y275

Research Use Only