Product Description

Human Barbiturates ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8382-48, ELK8382-96, ELK8382-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Barbituates

Assay Type: Competitive Inhibition

Sensitivity: 0.1 ng/mL

Standard: 25 ng/mL

Detection Range: 0.4-25 ng/mL

Sample Type: Plasma, Serum, Urine

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Barbitues. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Barbitues. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Barbitues in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only