Product Description

Human C5b (Complement Fragment 5b) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8530-48, ELK8530-96, ELK8530-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Complement Fragment 5b

Reactivity: Human

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Metabolic Pathway, Apoptosis, Infection Immunity

Assay Length: 3.5h

Sensitivity: 0.481 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Research Use Only