Human CA15-3 (Carbonhydrate Antigen 15-3) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0245MS-48, ELK0245MS-96, ELK0245MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CA15-3
Assay Type: Sandwich
Sensitivity: 0.49 U/mL
Standard: 100 U/mL
Detection Range: 1.57-100 U/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only