Human carboxyhemoglobin,HbCO ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8401-48, ELK8401-96, ELK8401-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: HbCO
Assay Type: Competitive Inhibition
Sensitivity: 2.37 ng/mL
Standard: 2000 ng/mL
Detection Range: 31.25-2000 ng/mL
Sample Type: Serum, plasma, other biological fluids
Assay Length: 2h
Research Areas: Enzyme and Kinase; Cardiovascular biology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HbCO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HbCO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HbCO in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only