Product Description

Human CASP9 (Caspase 9) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1530-48, ELK1530-96, ELK1530-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASP9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASP9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: APAF3, ICE-LAP6, MCH6, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 9, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Enzyme and Kinase, Apoptosis, Tumor Immunity, Infection Immunity

Assay Length: 3.5h

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: P55211

Research Use Only