Product Description

Human CAST (Calpastatin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK11217-48, ELK11217-96, ELK11217-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAST in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: BS17, Calpain inhibitor, Sperm BS-17 component

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Neuro Science, Developmental Science

Assay Length: 3.5h

Sensitivity: 0.21 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

UniProt: P20810

Research Use Only