Product Description

Human CDHOB (Cadherin, Osteoblast) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1792-48, ELK1792-96, ELK1792-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CDH11; CAD11; OB; OSF4; Cadherin 11 Type 2; OB-Cadherin

Assay Type: Sandwich

Sensitivity: 0.114 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: CD and Adhesion molecule; Tumor immunity; Bone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CDHOB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CDHOB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CDHOB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CDHOB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P55287

Research Use Only