Product Description

Human cGMP (Cyclic Guanosine Monophosphate) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8617-48, ELK8617-96, ELK8617-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human cGMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human cGMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: c-GMP, 3', 5'-Cyclic GMP, Cyclic 3', 5'-GMP, Guanosine 3', 5'-Cyclic Phosphate

Reactivity: Human

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Signal Transduction, Metabolic Pathway

Assay Length: 2h

Sensitivity: 43 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Research Use Only