Product Description

Human CK-18 (Cytokeratin 18) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8576-48, ELK8576-96, ELK8576-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Cell proliferation inducing gene 46 protein , Cell proliferation inducing protein 46 , Cell proliferation-inducing gene 46 protein , CK 18 , CK-18 , CK18 , CYK 18 , CYK18 , Cytokeratin 18 , Cytokeratin endo B , Cytokeratin-18 , K 18 , K18 , K1C18_HUMAN , KA18 , Keratin 18 , Keratin 18, type I , Keratin D , keratin, type I cytoskeletal 18 , Keratin-18 , Krt18

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cancer Of The Liver, Viral Hepatitis

Assay Length: 3.5h

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Research Use Only