Human CK 18-M65 (Cytokeratin 18-M65) ELISA Kit - ELK10045

Human CK 18-M65 (Cytokeratin 18-M65) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10045-48, ELK10045-96, ELK10045-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: K18-M65; CK-18-M65; CYK18-M65

Assay Type: Sandwich

Sensitivity: 29.8 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Cancer of the liver; Viral hepatitis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only