Human CoII2-1 (Degradation Product of Type-II Collagen) ELISA Kit - ELK0096

Human CoII2-1 (Degradation Product of Type-II Collagen) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0096-48, ELK0096-96, ELK0096-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: CoII2-1

Assay Type: Sandwich

Sensitivity: 18.2 pg/mL

Standard: 3000 pg/mL

Detection Range: 46.88-3000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CoII2-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CoII2-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CoII2-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CoII2-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only