Human CRH (Corticotropin Releasing Hormone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10412-48, ELK10412-96, ELK10412-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin
Assay Type: Competitive Inhibition
Sensitivity: 0.13 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Cardiovascular biology; Neuroscience; Hormone metabolism; Urology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P06850
Research Use Only