Product Description

Human cTnT/TNNT2 (Troponin T Type 2, Cardiac) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK3961MS-48, ELK3961MS-96, ELK3961MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CMH2, cTnT, TnTc, Cardiomyopathy, Hypertrophic 2, Cardiac muscle troponin T, TNNT2, Troponin T Type 2, Cardiac

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cardiovascular Biology

Assay Length: 3.5h

Sensitivity: 6.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

UniProt: P45379

Research Use Only