Human DEFb103A (Defensin Beta 103A) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4237-48, ELK4237-96, ELK4237-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: DEFB103; DEFB3; HBD-3; HBD3; HBP-3; HBP3; Beta-defensin 3; Defensin-like protein
Assay Type: Sandwich
Sensitivity: 19.59 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFb103A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFb103A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DEFb103A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DEFb103A in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P81534
Research Use Only