Product Description

Human ERK1/2 (Extracellular Signal Regulated Kinase 1/2) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0372-48, ELK0372-96, ELK0372-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Enzyme and Kinase, Apoptosis, Tumor Immunity

Assay Length: 3.5h

Sensitivity: 0.12 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Research Use Only