Human ES (Endostatin) ELISA Kit - ELK5401

Human ES (Endostatin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5401-48, ELK5401-96, ELK5401-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: FLJ27325; FLJ34914; KNO; KNO1; MGC74745;

Assay Type: Competitive Inhibition

Sensitivity: 1.72 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Tumor immunity; Cardiovascular biology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ES in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only