Product Description

Human ET-1 (Endothelin1) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2444MS-48, ELK2444MS-96, ELK2444MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: ET1, PPET1, Preproendothelin-1, Big endothelin-1, EDN1

Sensitivity: 2.69 pg/mL

Assay Type: Sandwich

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Endocrinology, Cardiovascular biology,

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P05305

Research Use Only