Product Description

Human F-actin (Filamentous Actin) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0113MS-48, ELK0113MS-96, ELK0113MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Fibrous Actin

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates

Assay Type: Sandwich

Research Areas: Signal Transduction, Developmental Science, Bone Metabolism

Assay Length: 3.5h

Sensitivity: 0.18 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Research Use Only