Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit - ELK10620

Human F13B (Coagulation Factor XIII B Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10620-48, ELK10620-96, ELK10620-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: F13-B; FXIIIB; Fibrin-stabilizing factor B subunit; Protein-glutamine gamma-glutamyltransferase B chain; Transglutaminase B chain

Assay Type: Competitive Inhibition

Sensitivity: 1.24 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: plasma

Assay Length: 2h

Research Areas: Signal transduction; Metabolic pathway; Hematology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human F13B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F13B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F13B in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P05160

Research Use Only