Product Description

Human GCK (Glucokinase) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1111-48, ELK1111-96, ELK1111-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GCK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GCK in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, Hexokinase-D, Hexokinase 4, Maturity Onset Diabetes Of The Young 2

Reactivity: Human

Sample Type: Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Metabolic Pathway, Endocrinology, Nutrition Metabolism

Assay Length: 3.5h

Sensitivity: 0.23 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

UniProt: P35557

Research Use Only