Product Description

Human GLUT12 (Glucose Transporter 12) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0860-48, ELK0860-96, ELK0860-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GLUT12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GLUT12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GLUT12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GLUT12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: SLC2A12, SLC2-A12, GLUT, Solute Carrier Family 2 Member 12, Facilitated Glucose Transporter, Glucose transporter type 12, erythrocyte/brain, HepG2 glucose transporter

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Metabolic Pathway, Endocrinology

Assay Length: 3.5h

Sensitivity: 0.067 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

UniProt: Q8TD20

Research Use Only